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minimal pcp2 l7 promoter  (Addgene inc)


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    Addgene inc minimal pcp2 l7 promoter
    (a) Mice were randomly divided into AAV5-hSyn-EGFP (control), NMDA (lesion) and <t>AAV8-Pcp2-hM3Dq-mCherry</t> (PC-M3) groups, followed by a series of behavioral tests. Clozapine-N-oxide (CNO, 1 mg/kg) was given to all the groups before each test. Mice were sacrificed 90 min after the final test (free social interaction, FSI) and c-Fos immunostaining was performed. (b) Examples of the sagittal view of cerebellar slices from the control, lesion and PC-M3 groups. Fluorescence in lobule IV/V was EGFP (control), DAPI (lesion) or mCherry (PC-M3). Zoom-in images are shown in bottom panels. In the control group, EGFP was found preferentially in granule cells and interneurons. In the lesion group, cell loss was seen in molecular layer (ML), Purkinje cell (PC) layer, and granular layer (GL). In the PC-M3 group, only PCs were labelled with mCherry. (c) Diagrams of the coronal view of cerebellar sections. Affected areas in lobule IV/V are highlighted in green (EGFP in control group), grid-shaped (lesions in lesion group) and red (mCherry in PC-M3 group), respectively. Numbers indicate distance relative to the bregma.
    Minimal Pcp2 L7 Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/minimal+pcp2+l7+promoter/pAAV-CaMKIIa-hM3D(Gq)-mCherry+(Plasmid+%2350476)/pmc08417139-83-0-7
    Average 94 stars, based on 80 article reviews
    minimal pcp2 l7 promoter - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Functional convergence of motor and social processes in lobule IV/V of the mouse cerebellum"

    Article Title: Functional convergence of motor and social processes in lobule IV/V of the mouse cerebellum

    Journal: Cerebellum (London, England)

    doi: 10.1007/s12311-021-01246-7

    (a) Mice were randomly divided into AAV5-hSyn-EGFP (control), NMDA (lesion) and AAV8-Pcp2-hM3Dq-mCherry (PC-M3) groups, followed by a series of behavioral tests. Clozapine-N-oxide (CNO, 1 mg/kg) was given to all the groups before each test. Mice were sacrificed 90 min after the final test (free social interaction, FSI) and c-Fos immunostaining was performed. (b) Examples of the sagittal view of cerebellar slices from the control, lesion and PC-M3 groups. Fluorescence in lobule IV/V was EGFP (control), DAPI (lesion) or mCherry (PC-M3). Zoom-in images are shown in bottom panels. In the control group, EGFP was found preferentially in granule cells and interneurons. In the lesion group, cell loss was seen in molecular layer (ML), Purkinje cell (PC) layer, and granular layer (GL). In the PC-M3 group, only PCs were labelled with mCherry. (c) Diagrams of the coronal view of cerebellar sections. Affected areas in lobule IV/V are highlighted in green (EGFP in control group), grid-shaped (lesions in lesion group) and red (mCherry in PC-M3 group), respectively. Numbers indicate distance relative to the bregma.
    Figure Legend Snippet: (a) Mice were randomly divided into AAV5-hSyn-EGFP (control), NMDA (lesion) and AAV8-Pcp2-hM3Dq-mCherry (PC-M3) groups, followed by a series of behavioral tests. Clozapine-N-oxide (CNO, 1 mg/kg) was given to all the groups before each test. Mice were sacrificed 90 min after the final test (free social interaction, FSI) and c-Fos immunostaining was performed. (b) Examples of the sagittal view of cerebellar slices from the control, lesion and PC-M3 groups. Fluorescence in lobule IV/V was EGFP (control), DAPI (lesion) or mCherry (PC-M3). Zoom-in images are shown in bottom panels. In the control group, EGFP was found preferentially in granule cells and interneurons. In the lesion group, cell loss was seen in molecular layer (ML), Purkinje cell (PC) layer, and granular layer (GL). In the PC-M3 group, only PCs were labelled with mCherry. (c) Diagrams of the coronal view of cerebellar sections. Affected areas in lobule IV/V are highlighted in green (EGFP in control group), grid-shaped (lesions in lesion group) and red (mCherry in PC-M3 group), respectively. Numbers indicate distance relative to the bregma.

    Techniques Used: Immunostaining, Fluorescence

    Related Articles

    Expressing:

    Article Title: Functional convergence of motor and social processes in lobule IV/V of the mouse cerebellum
    Article Snippet: AAV8-Pcp2-hM3Dq-mCherry (titer >4.0×10 13 ) was prepared by the University of Minnesota Viral Vector and Cloning Core following standard packaging procedures. .. Minimal Pcp2/L7 promoter was inserted into pAAV-CaMKIIa-hM3D(Gq)-mCherry (Addgene #50476, a gift from Dr. Bryan Roth) to confer specific expression of a human M3 muscarinic receptor (hM3Dq) to PCs (PC-M3) [ 31 , 37 ]. ..



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    Addgene inc minimal pcp2 l7 promoter
    (a) Mice were randomly divided into AAV5-hSyn-EGFP (control), NMDA (lesion) and <t>AAV8-Pcp2-hM3Dq-mCherry</t> (PC-M3) groups, followed by a series of behavioral tests. Clozapine-N-oxide (CNO, 1 mg/kg) was given to all the groups before each test. Mice were sacrificed 90 min after the final test (free social interaction, FSI) and c-Fos immunostaining was performed. (b) Examples of the sagittal view of cerebellar slices from the control, lesion and PC-M3 groups. Fluorescence in lobule IV/V was EGFP (control), DAPI (lesion) or mCherry (PC-M3). Zoom-in images are shown in bottom panels. In the control group, EGFP was found preferentially in granule cells and interneurons. In the lesion group, cell loss was seen in molecular layer (ML), Purkinje cell (PC) layer, and granular layer (GL). In the PC-M3 group, only PCs were labelled with mCherry. (c) Diagrams of the coronal view of cerebellar sections. Affected areas in lobule IV/V are highlighted in green (EGFP in control group), grid-shaped (lesions in lesion group) and red (mCherry in PC-M3 group), respectively. Numbers indicate distance relative to the bregma.
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    minimal purkinje cell-specific pcp2/l7 promoter  (Signal Research)
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    (a) Mice were randomly divided into AAV5-hSyn-EGFP (control), NMDA (lesion) and <t>AAV8-Pcp2-hM3Dq-mCherry</t> (PC-M3) groups, followed by a series of behavioral tests. Clozapine-N-oxide (CNO, 1 mg/kg) was given to all the groups before each test. Mice were sacrificed 90 min after the final test (free social interaction, FSI) and c-Fos immunostaining was performed. (b) Examples of the sagittal view of cerebellar slices from the control, lesion and PC-M3 groups. Fluorescence in lobule IV/V was EGFP (control), DAPI (lesion) or mCherry (PC-M3). Zoom-in images are shown in bottom panels. In the control group, EGFP was found preferentially in granule cells and interneurons. In the lesion group, cell loss was seen in molecular layer (ML), Purkinje cell (PC) layer, and granular layer (GL). In the PC-M3 group, only PCs were labelled with mCherry. (c) Diagrams of the coronal view of cerebellar sections. Affected areas in lobule IV/V are highlighted in green (EGFP in control group), grid-shaped (lesions in lesion group) and red (mCherry in PC-M3 group), respectively. Numbers indicate distance relative to the bregma.
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    Image Search Results


    (a) Mice were randomly divided into AAV5-hSyn-EGFP (control), NMDA (lesion) and AAV8-Pcp2-hM3Dq-mCherry (PC-M3) groups, followed by a series of behavioral tests. Clozapine-N-oxide (CNO, 1 mg/kg) was given to all the groups before each test. Mice were sacrificed 90 min after the final test (free social interaction, FSI) and c-Fos immunostaining was performed. (b) Examples of the sagittal view of cerebellar slices from the control, lesion and PC-M3 groups. Fluorescence in lobule IV/V was EGFP (control), DAPI (lesion) or mCherry (PC-M3). Zoom-in images are shown in bottom panels. In the control group, EGFP was found preferentially in granule cells and interneurons. In the lesion group, cell loss was seen in molecular layer (ML), Purkinje cell (PC) layer, and granular layer (GL). In the PC-M3 group, only PCs were labelled with mCherry. (c) Diagrams of the coronal view of cerebellar sections. Affected areas in lobule IV/V are highlighted in green (EGFP in control group), grid-shaped (lesions in lesion group) and red (mCherry in PC-M3 group), respectively. Numbers indicate distance relative to the bregma.

    Journal: Cerebellum (London, England)

    Article Title: Functional convergence of motor and social processes in lobule IV/V of the mouse cerebellum

    doi: 10.1007/s12311-021-01246-7

    Figure Lengend Snippet: (a) Mice were randomly divided into AAV5-hSyn-EGFP (control), NMDA (lesion) and AAV8-Pcp2-hM3Dq-mCherry (PC-M3) groups, followed by a series of behavioral tests. Clozapine-N-oxide (CNO, 1 mg/kg) was given to all the groups before each test. Mice were sacrificed 90 min after the final test (free social interaction, FSI) and c-Fos immunostaining was performed. (b) Examples of the sagittal view of cerebellar slices from the control, lesion and PC-M3 groups. Fluorescence in lobule IV/V was EGFP (control), DAPI (lesion) or mCherry (PC-M3). Zoom-in images are shown in bottom panels. In the control group, EGFP was found preferentially in granule cells and interneurons. In the lesion group, cell loss was seen in molecular layer (ML), Purkinje cell (PC) layer, and granular layer (GL). In the PC-M3 group, only PCs were labelled with mCherry. (c) Diagrams of the coronal view of cerebellar sections. Affected areas in lobule IV/V are highlighted in green (EGFP in control group), grid-shaped (lesions in lesion group) and red (mCherry in PC-M3 group), respectively. Numbers indicate distance relative to the bregma.

    Article Snippet: Minimal Pcp2/L7 promoter was inserted into pAAV-CaMKIIa-hM3D(Gq)-mCherry (Addgene #50476, a gift from Dr. Bryan Roth) to confer specific expression of a human M3 muscarinic receptor (hM3Dq) to PCs (PC-M3) [ 31 , 37 ].

    Techniques: Immunostaining, Fluorescence